The report said these centres, including beauty parlours, had been receiving visits in recent weeks from ministry officials posing as customers.
It said they were there to document evidence that the operators were flouting the law after making handsome profits from the ozone therapy.
NST said the directive followed a blanket ban on the commercial use of ozone therapy in the country, as announced by Health Minister Dr S Subramaniam on May 3.
It quoted deputy director-general of Health (medical) Dr S Jeyaindran as saying offenders would be prosecuted.
“We have given them six months to voluntarily opt out from the market and effective July 1, ozone therapy is considered an illegal procedure.
“Any medical practitioner who provides the procedure is breaching the Medical Device Act because they are using an unregistered medical machine.
“If they are non-medical practitioners, then they are breaking even more regulations because they cannot use needles or syringes, which they need to perform such treatments.”
He said the ministry’s Medical Device Authority (MDA) would also act based on public complaints.
Among risks associated with the treatment is death from air embolism when the needles are inserted into blood vessels.
Jeyaindran said there was no evidence to support claims of the treatment’s effectiveness.
The report said at least 40 centres in the Klang Valley were offering the ozone treatment now.
Some centres charge as much as RM800 to RM1,000 for such treatment for a single session to cleanse the blood of impurities and cholesterol.
Some centres are said to employ full-time qualified nurses to carry out the therapy and monitor the patients during the procedure.
The ministry is contacting the Malaysian Communications and Multimedia Commission to close websites promoting ozone therapy.